Perturbation analysis:

We will perform perturbation analyses in HMECs on the extended network with amongst other RNAi technologies. We will, after perturbation, measure the molecular state of the network across time. These perturbation analyses will generate a training data set for model construction.

Network state to phenotype mapping:

We will link the state of the Extended network to clinically relevant cellular phenotypes. We will record cellular phenotypes associated with PI3K and MAPK activation using our high content screening platform.